comparison between rt- pcr and viral isolation for detection of h9 subtype of avian influenza viruses

Authors

مهدی وصفی مرندی

گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران مهدی وصفی مرندی

گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران مهدی رزازیان

دامپزشک بخش خصوصی تهران

abstract

during 2004-2006, a total number of 22 tissue samples obtained from poultry flocks suspected to respiratory diseases submitted to avian virology laboratory of faculty of veterinary medicine, were prepared for avian influenza virus (aiv) isolation in embryonated chicken eggs, according to the standard method. rt-pcr was established on tissue samples using specific primers for h9 gene. amplified pcr products were 435 bp in length. analytical sensitivity of the rt-pcr was 103.5 eid50 and sensitivity, specificity and correlation rate compared with virus isolation, were 100%, 94% and 95%, respectively. the results showed that the rt-pcr assay using h9 gene specific primers, directly on tissue samples, could be used in rapid detection and subtyping of h9 aivs as a useful alternative to virus isolation assay.

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Journal title:

تحقیقات دامپزشکی

جلد ۶۳، شماره ۱، صفحات ۱۷-۲۲

Keywords

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